Supplementary Figure 1: Impaired sensory-evoked GCaMP6s dynamics in SSC of Syngap1 mice.

(a) Example wide-field ΔF/F images superimposed over craniotomy pictures of WT and Het mice. (b-d) Traces of individual (b) and averaged (c) GCaMP6s responses, as well as a scatter plot showing the integrated ΔF/F signal amplitude (d) in response to 5 pulses at 5 Hz (Student’s t-test t(12)=4.09 p=0.0015). (e-g) Average time courses of GCaMP6s dynamics in response to a single whisker stimulation (e), 5 pulses at 5 Hz (f) and 5 pulses at 1 Hz (g) stimulation protocols. (h) Scatter plot showing ratio of fourth pulse to first pulse in 5 pulse at 1 Hz paradigm (Student’s t-test t(12)=4.16 p=0.0013). Peak (i, j) and integrated (k, l) ΔF/F responses for increasing number of pulses at 10 Hz (j, l) and increasing frequency of pulses at 5 pulses (i, k) (2-way RM-ANOVA : (i) F(1,12)=3.90 p=0.07 for genotype and F_(2,24)=7.44 p=0.003 for genotype and frequency interaction; (j) F(1,12)=2.27 p=0.16 for genotype and F_(4,48)=2.92 p=0.031 for genotype and frequency interaction; (k) F(1,12)=7.11 p=0.02 for genotype and F_(2,24)=4.92 p=0.01 for genotype and frequency interaction; (l) F(1,12)=4.22 p=0.06 for genotype and F_(4,48)=5.96 p=0.001 for genotype and frequency interaction). Open circles represent animal means, black lines or closed circles indicate population means and error bars or shaded areas indicate SEMs. Data were obtained n= 6 WT and n=8 Het mice from two cohorts of animals. All statistical tests were two-sided.