Supplemetary Figure 3: Regression correlation analysis with longitudinal imaging of microglial activity as well as fibrillar amyloidogenesis in individual mice.
From: Loss of TREM2 function increases amyloid seeding but reduces plaque-associated ApoE
a,b, Coronal and axial slices show serial TSPO (a) and Amyloid μPET (b) Z-score increases (versus C57BL6) from three to twelve months of age for APPPS1/Trem2+/+ and APPPS1/Trem2-/- mice. APPPS1/Trem2-/- mice indicate decreased microglial activity, most pronounced at twelve months of age, and higher fibrillar amyloidogenesis at three and six months of age when compared to APPPS1/Trem2+/+ mice. c,d, Spaghetti plots show individual longitudinal time courses of cortical microglial activity (c) and fibrillar amyloidogenesis (d) as assessed by in vivo μPET in APPPS1/Trem2+/+ (purple highlighted data point corresponds to representative image shown in e and f) and APPPS1/Trem2-/- mice (dark blue highlighted data point corresponds to representative image shown in a and b). e,f, x34/CD68/IBA1 co-stained microglia (n+/+ = 8 mice; n-/- = 7 mice) as well as (e) fibrillar and immunostained amyloid plaques (f) (n+/+ = 8 mice; n-/- = 7 mice) of twelve months old APPPS1 mice previously included in longitudinal TSPO and Amyloid μPET imaging. g,h, Regression correlation analysis of CD68-positive phagocytic microglia staining and TSPO μPET imaging (n+/+ = 5 mice, n-/- = 4 mice, P = 0.0026) (g) and x34-positive fibrillar Aβ staining and amyloid μPET imaging (n+/+ = 4 mice, n-/- = 4 mice, P = 0.0003) (h)in three months old APPPS1 mice. i,j, Regression correlation analysis of CD68 and TSPO μPET imaging (n+/+ = 10 mice, n-/- = 5 mice, p = 5.2E-5) (i)and x34 and Amyloid μPET imaging (n+/+ = 4 mice, n-/- = 4 mice, P = 0.0088) (j)in six months old APPPS1 mice. k,l Regression correlation analysis of CD68 and TSPO μPET imaging (n+/+ = 8 mice, n-/- = 7 mice, P = 1.0 x 10-6) (k) and x34 and Amyloid μPET imaging (n+/+ = 7 mice, n-/- = 7 mice, P = 2.0 × 10–5) (l) in twelve months old APPPS1 mice. Purple dots represent APPPS1/Trem2+/+ and blue dots indicate APPPS1/Trem2-/- mice.
