Supplementary Figure 2: TDP-43 knockdown in cultured hMNs.
From: ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair
a, RNAi strategy for TDP-43 knockdown in cultured motor neurons. b, Phase and red fluorescence micrographs of cultured hMNs 4 days after treatment with different siRNAs including scrambled siRNA conjugated to Alexa Fluor 555 (siRED). c, Flow-cytometric analysis of hMNs after treatment with different siRNAs. d, Relative levels of TDP-43 mRNA in motor neurons exposed to different siRNAs for 2, 4 or 6 days. Levels for each sample were normalized to GAPDH and expressed relative to the no transfection control. Data are displayed as mean of technical replicates with s.d. of replicates from n = 2 experiments. e, Immunoblot analysis of hMNs after RNAi treated with the indicated siRNAs. Each sample was normalized using GAPDH, and TDP-43 protein levels were calculated relative to the siRED-treated control sample. Similar results were obtained for these experiments in n = 3 independent experiments.
