Supplementary Figure 8: STMN2 expression is decreased in human motor neurons expressing TDP-43 from the AAVS1 locus.
From: ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair
a, AAVS1 gene targeting and genotyping strategy. b, Genotyping the AAVS1 locus after targeting demonstrates a single copy integration. Similar data were obtained for two independent experiments. c, qRT-PCR analysis of STMN2 in motor neurons differentiated from the HUES3 Hb9::GFP and the gene targeted lines expressing wildtype or mutant TDP-43. Data are displayed as mean with s.d. of technical replicates from n = 2 independent experiments (unpaired t-test, two-sided, P < 0.05).
