Supplementary Figure 9: Proteasome activity assays.
From: ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair
a, MG-132 neuronal survival experimental outline. b, Dose response curve for hMNs cultured with indicated concentrations of MG-132 for the indicated times. Data are displayed as technical replicates from n = 2 independent experiments and the lines represent the mean. Cells were viable after 1 day of treatment at all concentrations tested and lower concentrations were tolerated for more extended periods of time. Similar results have been obtained for n = 4 independent experiments. c, Following cleavage by the proteasome, substrate for luciferase is liberated, which allows for quantitative measurement of proteasome activity. Neurons treated with MG-132 show significantly decreased proteasome activity. Data are displayed as box and whisker plots showing min to max for technical replicate from n = 2 independent experiments (unpaired t-test, two-sided, P < 0.05).
