Supplementary Figure 5: Mitophagy induction protects against memory impairment in C. elegans AD models. | Nature Neuroscience

Supplementary Figure 5: Mitophagy induction protects against memory impairment in C. elegans AD models.

From: Mitophagy inhibits amyloid-β and tau pathology and reverses cognitive deficits in models of Alzheimer’s disease

Supplementary Figure 5

We evaluated memory defects in the AD nematodes using an aversive olfactory learning paradigm. To determine whether memory retention is dependent on mitophagy in the Aβ1–42 (CL2355) nematodes, we generated three mitophagy mutant strains, pink-1(tm1779);CL2355, pdr-1(gk488);CL2355, and dct-1(tm376);CL2355. a-c, NMN-, UA-, and AC-induced memory improvement depends differentially on PINK-1, PDR-1 and DCT-1 activity (n = 400 nematodes/group; n.s., p > 0.05, ****p < 0.0001; Two-way ANOVA followed by Tukey’s multiple comparisons test). d, Beneficial effects of NMN, UA, and AC on memory of Aβ1–42 expressing nematodes (CL2355) differentially depend on mitophagy genes. For all experiments, nematodes were treated with NMN (5 mM) and AC (1 mM) for 2 generations, and with UA (0.1 mM) from eggs to the day of experiment for one generation. Adult Day 1 nematodes were used for the memory assay. For all worm experiments, 2 to 4 independent experiments were performed. For all ‘dot-plot’ figures, center value represents mean and error bars represent s.e.m.

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