Supplementary Figure 10: Effects of sex difference on mitophagy-induction-induced benefits in two AD mouse models.
a-b, The APP/PS1 mice were treated with UA (200 mg/kg/day) or AC (30 mg/kg/day) by daily gavage for 2 months starting from 6 months of age, and then the Aβ1–42 and Aβ1–40 levels in the hippocampal region were detected using standard ELISA techniques. Data shown in changes of hippocampal Aβ1–42 (a) or Aβ1–40 (b) levels in male and female mice. For the mouse numbers in (a) and (b), n = 8 (4 males + 4 females) in WT (veh.), n = 8 (4 males + 4 females) in AD (veh.), n = 9 (5 males + 4 females) in AD (UA), and n = 8 (5 males + 3 females) in AD (AC). Center value represents mean and error bars represent s.e.m. (*p < 0.05, **p < 0.01, ***p < 0.001; One-way ANOVA). Sex difference of the data shown in Fig. 3f, g were reanalyzed here. c-f, effects of one-month UA treatment on memory performance in 3xTgAD mice. Thirteen-month old 3xTgAD mice were treated with UA (200 mg/kg/day) by daily gavage for 1 month. To investigate any sex difference, the data show in Fig 5i–l were further analyzed here. Contextual and cued fear conditioning test (c, d), object recognition test (e), and Y-maze test (f) were performed. For the mouse numbers using in c-f, n = n = 7 (5 males + 2 females) in WT (veh.), n = 7 (3 males + 4 females) in 3xTgAD (veh.), n = 7 (3 males + 4 females) in 3xTgAD (UA). Center value represents mean and error bars represent s.e.m. (*p < 0.05, **p < 0.01, ***p < 0.001; Two-sided Student’s t-test was used for the comparison between 2 groups, while One-way ANOVA was used to compare three groups.).
