Supplementary Figure 1: Characterization of postmortem hippocampal samples and iPSC-derived neurons from AD patients and neurologically normal controls. | Nature Neuroscience

Supplementary Figure 1: Characterization of postmortem hippocampal samples and iPSC-derived neurons from AD patients and neurologically normal controls.

From: Mitophagy inhibits amyloid-β and tau pathology and reverses cognitive deficits in models of Alzheimer’s disease

Supplementary Figure 1

a, Quantification of Western blot data of the indicated proteins. Data were shown in mean ± s.e.m. (n = 7 biologically independent samples from 7 AD patients or 7 healthy controls; *p < 0.05; Two-sided Student’s t-test). b, Levels of proteins related to mitochondrial homeostasis in postmortem hippocampal samples from AD patients and age-matched controls. The right panel shows quantified data between normal and AD groups. Experiments were repeated independently twice with similar results. c, Representative IHC images showing colocalization of TOMM20 (mitochondrial outer membrane protein) and LAMP2 (lysosomal protein) in postmortem hippocampal regions of AD patients and age-matched healthy controls (n = 3 samples/group). Experiments were repeated independently twice with similar results. d, (upper) Levels of neuronal markers in two AD patient iPSC-derived neuronal lines and a sex- and age-matched control. (lower) Western blotting showing levels of mitophagy-related proteins in a PSEN1 AD patient iPSC-derived neuronal lines and matched control. Experiments were performed once. e and f, Levels of proteins involved in mitochondrial function and dynamics, metabolism, DNA repair, and mitophagy in two AD patient iPSC-derived neuronal lines and a shared sex- and age-matched control. g, Quantification of Western blot data of the indicated proteins. Data are shown in mean ± s.e.m. (n = n = 3 independent experiments; n.s., p>0.05 and *p < 0.05, **p < 0.01, ***p < 0.001; One-way ANOVA). Experiments were performed three times. h, ATP levels in postmortem hippocampal samples from AD patients and age-matched controls. Data are shown in mean ± s.e.m. (n = 7 biologically independent samples; ***p < 0.001; Two-sided Student’s t-test). i, Quantification of autophagosomes and autolysosomes in the APP and control cells with the ptfLC3 plasmid, with data shown in mean ± s.e.m. (n = 20 neurons from 3 independent experiments; ***p < 0.001; Two-sided Student’s t-test). Full scans of all the blots are in Supplementary Note.

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