Supplementary Figure 2: Monitoring of neuronal mitophagy in vivo.

Transgenic nematodes expressing mtRosella biosensor in neuronal cells treated with NMN, UA, and AC. Decreased GFP/DsRed ratio of mtRosella indicates neuronal mitophagy stimulation. DCT-1, PDR-1 and PINK-1 were required for neuronal mitophagy induction in response to UA, NMN and AC treatment. Scale bars, 20 μm. Quantitative data shown in Fig. 2d. Experiments were repeated independently three times with similar results.