Supplementary Figure 4: The optimized doses of NMN, UA, and AC do not induce general macro-autophagy in C. elegans. | Nature Neuroscience

Supplementary Figure 4: The optimized doses of NMN, UA, and AC do not induce general macro-autophagy in C. elegans.

From: Mitophagy inhibits amyloid-β and tau pathology and reverses cognitive deficits in models of Alzheimer’s disease

Supplementary Figure 4

a-b, Supplementation with NMN (5 mM), UA (0.1 mM), and AC (1 mM) does not alter general autophagy levels in C. elegans neurons. Representative images showing transgenic nematodes expressing the autophagosomal marker LGG-1 fused with GFP (a) or DsRed (b) (n = 30 neuronal cells/group; ***p < 0.001; one-way ANOVA followed by Sidak’s multiple comparisons test). Scale bars, 5 μm. c-h, C. elegans transgenic animals expressing full-length plgg-1GFP::LGG-1, plgg-2GFP::LGG-2 and patg-18ATG-18::GFP fusion proteins indicative of autophagic activity treated with NMN (5 mM), UA (0.1 mM), and AC (1 mM) (n = 30 nematodes/group; ***p < 0.001; one-way ANOVA followed by Sidak’s multiple comparisons test). Several tissues were evaluated, including intestines (c-d; Scale bars, 40 μm), embryos (e; Scale bars, 10 μm), muscles (f; Scale bars, 20 μm), and neurons (g; Scale bars, 5 μm). Starvation-induced autophagy used as an internal positive control. Error bars, ± s.e.m.

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