Supplementary Fig. 4: Dorsal–ventral division of the mPFC; differential cortical innervation of the divisions.

(a) Based on the anatomical distribution of the starter neurons within the mPFC subregions, the animals were divided into a dorsal group (pink; n = 10 animals), and a ventral group (green; n = 7 animals), respectively (see Methods for details). (b) The density (normalized) of cortical RV-eGFP labeled input neurons along the A-P axis in the dorsal (pink) and ventral (green) groups (RV-eGFP labeled neurons in the mPFC are excluded). Bin width: 0.16 mm. (c) Detailed analysis of the cortical input pattern for the dorsal (pink), and the ventral (green), animal groups. A two-sided Student’s t-test was used to generate P values. No correction for multiple comparisons was applied. See Supplementary Table 2 for statistical values. (d) Spearman Correlation matrix and hierarchal clustering (average method) investigating the co-variance of the proportion of input neurons in the regions of the isocortex. Red lettering; mPFC subregions, black lettering; cortical regions, pink lettering; cortical regions with a significantly higher proportion input to the dorsal mPFC, green lettering; cortical regions with a significantly higher proportion input to the ventral mPFC. Data from 17 animals, sign. level 0.05. Red: positive correlation, blue: negative correlation. Data from dorsal: n = 10, ventral: n = 7 animals / group in (b-c). *P < 0.05, data shown as mean ± s.e.m, circles denote individual animals in (c).