Supplementary Fig. 5: Characterization of Bsn expression.
From: Bassoon proteinopathy drives neurodegeneration in multiple sclerosis
a, Analysis of BSN expression in human MS microarray data from Han, M. H. et al. J Exp Med 2012. b, Gene expression analysis of Bsn in an independent EAE TRAP cohort (n = 3 per group). MN, motor neurons. iMN, inflamed motor neurons. Student’s t-test, two-tailed: n = 4 MN versus n = 3 iMN samples each pooled from 3 mice, t(5) = 2.695, P = 0.0431. Bars show mean values plus SEM. c, Immunohistochemical stainings of Bsn with quantification in spinal cord sections of healthy and chronic EAE mice at day 30 post immunization. Co-staining for neurons (NeuN). Student’s t-test, two-tailed: n = 3 mice per group, t(4) = 4.062, P = 0.0153. Bars show mean values plus SEM. MFI, mean fluorescence intensity. Scale bar, 20 µm. d, Immunohistochemical staining of Bsn in spinal cord sections of animals immunized with complete Freund’s adjuvant but without MOG35–55 at day 15 post immunization. Co-staining for neurons (NeuN). Experiment was repeated two times with similar results. Scale bar, 20 µm. e, Immunohistochemical staining of Bsn in spinal cord sections of EAE animals at day 15 post immunization. Co-staining for neurons (NeuN). Experiment was repeated three times with similar results. Scale bar, 20 µm. f, Immunohistochemical stainings of synapsin 1/2 in cervical spinal cord sections of healthy and chronic EAE mice. Co-staining for neurons (NeuN) and bassoon (Bsn). Experiment was repeated two times with similar results. Scale bar, 20 µm. *P < 0.05.
