Supplementary Fig. 8: Neuroaxonal numbers and immune cell infiltration in Bsn^−/−and IU1 treatment EAE.

a, Quantification of neurofilament-positive axons in the dorsal columns of cervical spinal cord sections of wildtype and Bsn^−/− animals without EAE induction. Student’s t-test, two-tailed: n = 3 mice per group, t(4) = 1.165, P = 0.3086. Bars show mean values plus SEM. b, Quantification of NeuN-positive neurons in cervical spinal cord sections of wildtype and Bsn^−/− animals without EAE induction. Student’s t-test, two-tailed: n = 3 mice per group, t(4) = 0.01243, P = 0.9907. Bars show mean values plus SEM. c, Gating strategy of CNS-infiltrating T cells. d, T cell subset composition in CNS infiltrates of wildtype and Bsn^−/− EAE animals at day 15 after immunization. Two-way ANOVA with Sidak’s post hoc test: n = 3 mice per group, F(2,12) = 0.0060, P = 0.9940; Sidak’s post hoc test: CD8⁺ T cells, WT versus Bsn^−/−: P = 0.9998; CD4⁺ T cells, WT versus Bsn^−/−: P = 0.9998; CD4⁺Foxp3⁺ Treg, WT versus Bsn^−/−: P > 0.9999. Bars show mean values plus SEM. e, T cell subset composition in CNS infiltrates of vehicle and IU1-treated EAE animals at day 15 after immunization. Two-way ANOVA with Sidak’s post hoc test: n = 3 mice per group, F(2,12) = 6.666, P = 0.0113; Sidak’s post hoc test: CD8⁺ T cells, vehicle-treated versus IU1-treated: P = 0.0778; CD4⁺ T cells, vehicle-treated versus IU1-treated: P = 0.0778; CD4⁺Foxp3⁺ Treg, vehicle-treated versus IU1-treated: P = 0.7514.Bars show mean values plus SEM.