Supplementary Fig. 1: Chat-L10a-eGFP expression validation and sample similarity analysis.
From: Bassoon proteinopathy drives neurodegeneration in multiple sclerosis
a, Representative immunohistochemical stainings of astrocytes (GFAP), vessels (CD31), microglia (Iba1), immune cells (CD45), oligodendrocytes (CNPase) and neurons (NeuN) co-localized with eGFP in cervical spinal cord sections of Chat-L10a-eGFP mice. Experiment was repeated two times with similar results. Scale bar, 50 µm. b, Number of motor neurons in cervical spinal cord sections of healthy (n = 3) and acute EAE (n = 4) Chat-L10a-eGFP mice with quantification. Student’s t-test, one-tailed: n = 3 healthy versus n = 4 EAE mice, t(5) = 2.440, P = 0.0293. Bars show mean values plus SEM. Scale bar, 100 µm. c, Principal component analysis including the top 500 variable genes. Experimental groups: spinal cord (SC), inflamed spinal cord (iSC), motor neurons (MN), inflamed motor neurons (iMN). d, Heatmap of Eeuclidean sample distances with unsupervised hierarchical clustering. Colors of experimental groups as in (c). *P < 0.05; **P < 0.01.
