Supplementary Figure 3: Clustering analysis and spatial locations of clusters. | Nature Neuroscience

Supplementary Figure 3: Clustering analysis and spatial locations of clusters.

From: Single-cell activity tracking reveals that orbitofrontal neurons acquire and maintain a long-term memory to guide behavioral adaptation

Supplementary Figure 3

a. Schematic of the clustering analysis (Methods) b. Scree plot of the percentage explained variance per principal component, showing the number of principal components retained (Methods). c. Individual retained principal components, showing response vectors to both CS+ and CS-. d. Clustering stability analysis by subsampling different fractions of trials to recalculate the PSTHs used for clustering. Adjusted Rand Index provides an index of stability for the clustering, and also reflects trial-to-trial variability in responses. Even a 100% subsampling shows instability due to the stochastic nature of spectral clustering. Measure of center is mean and error bars represent standard error of the mean. n=20 randomization repeats were run in each case.. e. Locations of individual cells for each cluster. Locations of all cells are shown in black behind the locations of cells within each cluster. The dorsal-ventral axis was split in half into a “dorsal plane” and a “ventral plane” for display purposes. The shift in mean location for each cluster with respect to the mean location of all cells is shown in Fig. 1l.

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