Supplementary Figure 9: Response evolution over acquisition.

a. CS+ trace response evolution of all neurons, including those with sigmoidal evolution, over acquisition. (Top row) The response evolution for every longitudinally-tracked neuron across behavioral acquisition is plotted for the learning-related clusters. Since the number of sessions until acquisition was different for different animals, the x-axis represents normalized trial block. In other words, the total number of trial blocks during acquisition (similar to Fig. 4a) was rescaled to contain the 15 normalized blocks on the x-axis. This rescaling was done purely for visualization and not for analysis. (Bottom row) Same as the top row but for neurons whose response evolution fits a sigmoidal model (Methods, Fig. 4c). b. Same plot as in Fig. 4b for reward responses, showing that even reward responses exhibit considerable evolution during acquisition. c. Same as Fig. 4g for reward responses, showing that cluster 1 shows earlier reward response evolution for both OFC-CaMKII and OFC-VTA neurons. d. Mean lag of all neurons from a cluster recorded within each individual animal for onset, trace and reward responses. The size of each dot is proportional to the number of neurons recorded within that animal from the given cluster. Hence, OFC-VTA dots are smaller due to the overall lower numbers of neurons recorded. Despite the fact that both OFC-CaMKII and OFC-VTA are completely independent groups, the distribution of means is quite similar between these groups per cluster. Since numbers of neurons per cluster is not a priori expected to be identical across animals due to possible anatomical variation, pooled data could in principle be contributed to more by some animals. Nevertheless, the independent replication between OFC-CaMKII and OFC-VTA groups of mean lags per animal suggests that the patterns uncovered here are robust.