Supplementary Figure 14: Stability of CS+ trace responses after learning.

a. A similar plot as in Supplementary Fig 13a is shown for longitudinally-tracked neurons across Day 1, Day before Trained, Trained, 50%, Background and After Background (same as Day before extinction) sessions. Comparison across clusters shows that the response profiles of neurons within a cluster retains the same response profile across sessions after learning and are different from the responses of all other clusters. b. Similar decoding analyses as in Fig. 7b done by training the decoder on the Trained session and testing on other sessions. c. An alternative analysis to the decoding stability analysis is to test if there is significant correlation in neuronal responses between different pairs of sessions. The Pearson’s correlation is shown for neuronal responses across all neurons (pooled across clusters) for different session pairs for both OFC-CaMKII and OFC-VTA neurons. These show that the correlation between session pairs becomes high only after learning. In order to remove the effect of between-animal variability from within-animal variability for cross-correlation, the responses within every animal were whitened (mean-subtracted and divided by standard deviation) prior to pooling between animals. This prevents issues similar to Simpson’s paradox. Measure of center in all panels is the mean and error bars represent standard error of the mean. * represents p < 0.05 (see Supplementary Table 1 for exact p values, sample sizes and tests).