Supplementary Figure 4: CLEM workflow.
From: Lewy pathology in Parkinson’s disease consists of crowded organelles and lipid membranes
Correlative light and electron microscopy (CLEM) is often used to localize specific molecules of interest within the complex and diverse biological landscape of cells and tissues, typically via genetically encoded fluorescent or enzymatic markers. Light microscopy is first used to visualize wide-field images with limited resolution, essentially providing a map to the labeled structures of interest. Such a map is then used to guide to the structure of interest for higher-resolution visualization by electron microscopy at a smaller imaging window. The general sequence of steps taken to achieve this for PD brain tissue sections is shown. EM = electron microscopy; LM = light microscopy.
