Supplementary Figure 10: Subcellular distribution of aSyn and organelle markers within Lewy pathology without a p-aSyn positive outer layer.
From: Lewy pathology in Parkinson’s disease consists of crowded organelles and lipid membranes
STED microscopy showing distribution of (a) marker for phosphorylated aSyn (pS129), (b) marker for mitochondria (porin VDAC1), (c) marker for lysosomes (LAMP1), (d) overlay of ‘a-c’, (e) higher magnification view of the edge of the aSyn-immunopositive inclusion shown in ‘d’ (f–i) Same STED microscopy and markers as in ‘a-d’, but a different inclusion, showing empty vacuoles that may represent autophagic vacuolar-like structures reminiscent of CLEM (Supplementary Fig. 12), (j) higher magnification view of center of the inclusion shown in ‘i’. (k–n) Same STED microscopy and markers as in ‘a-d,’ but a LN, (o) higher magnification view of the LN as in ‘n.’. Images are representative across 14 PD donors for Lewy structures without the p-aSyn outer layer. Scale bars: d, i, n = 2 µm; e, j, o = 1 µm.
