Supplementary Figure 11: Co-localization of lipids with aSyn in Lewy pathology.
From: Lewy pathology in Parkinson’s disease consists of crowded organelles and lipid membranes
Confocal fluorescence light microscopy projected image stacks of snap-frozen 10µm-thick tissue showing aSyn-immunopositive inclusions in the (a–c) hippocampal CA2 region of Donor A-PD, and (d–f) SN of Donor B-PD. Inclusions immunopositive for aSyn are visualized in green (LB509 antibody), lipid-rich structures are visualized in red by Nile Red staining, and nuclei are visualized in blue by DAPI. Column i = aSyn (green), nuclei (blue); Column ii = lipids (red), nuclei (blue); Column iii = overlay of aSyn (green), lipids (red), and nuclei (blue); Column iv = co-localization of aSyn and lipids (yellow). Scale bars: a, d = 20 µm; b, c, e, f = 5 µm.
