Supplementary Figure 12: Lipid and protein distributions in Lewy pathology detected by label-free CARS or FTIR imaging combined with correlative immunofluorescence CLSM for aSyn.
From: Lewy pathology in Parkinson’s disease consists of crowded organelles and lipid membranes
In Donor A-PD, CA2. (a) CARS image of lipids in an aSyn-immunopositive inclusion in PD brain tissue, recorded at 2850 cm-1. (b) Projected confocal immunofluorescence stack showing the same area, after immunostaining for aSyn (LB509). (c) Overlay of the CARS and aSyn immunofluorescence data shown in ‘a’ and ‘b’. (d) CARS intensity distribution profiles for lipids and proteins within the area, showing high peaks in the region of the LB. (e) FTIR image of lipids in an aSyn-immunopositive inclusion in PD brain tissue. (f) Projected confocal immunofluorescence stack showing the same area, immunostained for aSyn (LB509). (g) Overlay of the FTIR and aSyn immunofluorescence data shown in e and f. (h) FTIR intensity distribution profiles of lipids and proteins within the inclusion. Scale bars: 20 µm.
