Supplementary Fig. 1: Intrathecal Chase Infusions in the Pig Do Not Degrade Gray Matter Chondroitin Sulfate Proteoglycans.
From: Chondroitinase improves anatomical and functional outcomes after primate spinal cord injury
(a) 2B6 labeling of digested stubs of chondroitin sulfate proteoglycans (CSPGs) in transverse spinal cord sections of the porcine spinal cord at C8. Following intrathecal Chase infusions, there is an approximate 1 mm rim of CSPG degradation in the peripheral white matter of Chase-treated subjects. (b) Saline-infused control. (c) Wisteria Floribunda Agglutinin (WFA) labeling of peri-neuronal net CSPGs in gray matter reveals intact peri-neuronal net CSPGs in all Chase-infused subjects, which appear identical to (d) saline-infused controls. Labeling experiments included 6 Chase-infused and 1 saline-infused animals. Scale bars: 500 μm.
