Supplementary Fig. 2: Parenchymal Integrity at Chase Injection Sites – Nissl.
From: Chondroitinase improves anatomical and functional outcomes after primate spinal cord injury
Intraparenchymal injections of Chase in the monkey spinal cord do not cause extensive damage or inflammation. Images of transverse sections very near injection sites at C8-T1. Left column of images is from a short-term Chase subject (2 wks post injection, 6 wks post SCI); middle column is from a saline-injected subject (4 mos post injection, 5 mos post SCI); right column is from a Chase-injected subject (4 mos post injection, 5 mos post SCI). (A) Labeling for Nissl substance reveals mild hypercellularity along the injection track (arrowheads) 2 weeks after Chase injection. (B-C) By 4 months after injections, there are no evident differences in Nissl stains comparing saline and Chase injection sites. (D-F) Higher magnifications of sections in A-C. (G-I) Further magnification of panels D-F. Labeling experiments included 2 short-term Chase subjects, 4 saline-injected subjects, and 6 Chase-injected subjects. Scale bars: A-C, 500 μm; D-F, 100 μm; G-I, 50 μm.
