Supplementary Fig. 3: Parenchymal Integrity at Chase Injection Sites – CD8, GFAP, Motor Neurons.

Intraparenchymal injections of Chase in the monkey spinal cord do not cause extensive damage or inflammation. Images of transverse sections midway between injection sites at C8-T1. (A-C) CD8 labeling in gray matter reveals rare T-cells 2 weeks after Chase injection; these cells are not detected 4 months after injection. CD3 and CD45 labeling showed the same pattern (data not shown). (D-F) Labeling for GFAP (astrocytes) in gray matter shows no qualitative differences between short-term Chase, long-term saline, and long-term Chase. Immunolabeling experiments included 2 short-term Chase subjects, 4 saline-injected subjects, and 6 Chase-injected subjects. (G) Injections of either Chase or saline resulted in no loss of spinal motor neurons (MNs) on injected vs. uninjected sides of the spinal cord (expected proportion is 0.5). Moreover, there was no drop in MN numbers at the actual injection site, nor when comparing motor neuron numbers in Chase vs. control animals (Chase proportion: 0.53±0.04; Control proportion: 0.51±0.01; ANOVA: F_{(1, 0.00055)} = 0.11, P=0.75). N=6 Chase and N=5 controls. Lines show group means, bars show SEM, data points are individual tissue sections, 5-6 total sections per subject (up to one section per x-axis position). Scale bars: 100 μm.