Supplementary Figure 2: TRPV4-specific currents in TRPV4-P2A-ferritin-P2A-mCherry expressing 293T cells. | Nature Neuroscience

Supplementary Figure 2: TRPV4-specific currents in TRPV4-P2A-ferritin-P2A-mCherry expressing 293T cells.

From: Revaluation of magnetic properties of Magneto

Supplementary Figure 2

(a) Whole-cell patch-clamp recordings of outward (at 100 mV, blue trace) and inward (at -100mV, black trace) currents in 293T cells expressing control TRPV4-P2A-ferritin-P2A-mCherry in response to TRPV4 agonist GSK101 (10 nM) and antagonist GSK205 (10 µM). (b) Current-voltage relationships of control (black trace), the GSK101- (blue trace) and GSK205- (red trace) evoked responses in 293T cells expressing TRPV4-P2A-ferritin-P2A-mCherry. (c) Box charts showing quantification of the peak currents in 293T cells expressing TRPV4-P2A-ferritin-P2A-mCherry (Ctrl: -8.3±1.2 pA; GSK101: -48.5±7.8 pA; n=10, Z=-2.803, p<0.01, for inward currents at -100 mV; Ctrl: 13.1±1.7 pA; GSK101: 75.5±11.9 pA; n=10, Z=2.803, p<0.01, for outward currents at 100 mV).

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