Supplementary Figure 10: Defining in vivo burst and inter-burst events.

(a) Population Ca²⁺ events represented as two temporal traces: percentage of imaging field active (top) and number of AQuA event onsets (middle). Burst periods (pink) are defined from the top trace as periods when Ca²⁺ activity exceeds 1% of the active field of view (red dashed line, top), and exceeds 10% of the maximum number of event onsets (red dashed line, middle). Burst periods correlate with wheel velocity of the treadmill (bottom). (b) Burst onset is defined as the first frame in which 10% of the peak is exceeded and burst offset is defined as the last frame exceeding 10% of the peak. (c) The relationship between all interburst events’ total propagation distance and size, similar to the burst events plotted in Fig 4c. (n=6 mice).