Supplementary Figure 8: Endothelial cell enrichment. | Nature Neuroscience

Supplementary Figure 8: Endothelial cell enrichment.

From: Profiling the mouse brain endothelial transcriptome in health and disease models reveals a core blood–brain barrier dysfunction module

Supplementary Figure 8

A graphical representation of the key steps of the endothelial cell enrichment procedure used in this study. The regions of interests in health or disease experiments for brain, heart, lung, liver, kidney or spinal cord are identified in box A. The blue flow path (B) is the standard procedure. The violet flow path (C) adds the second enzymatic digestion step to dissociate remaining adherent cells used in the health “Brain endothelial” samples. The red flow path (D) adds the modifications for all peripheral organs. The green flow path (E) further adds modifications to the peripheral organ procedures for the activated beta-catenin studies.

Back to article page