Supplementary Figure 9: FACS gating strategy for endothelial cell enrichment. | Nature Neuroscience

Supplementary Figure 9: FACS gating strategy for endothelial cell enrichment.

From: Profiling the mouse brain endothelial transcriptome in health and disease models reveals a core blood–brain barrier dysfunction module

Supplementary Figure 9

Representative sample. (A) Gating for single cells with side scatter (SSC) and forward scatter (FSC). (B) Gating to exclude dead cells (DAPI+), and immune cells and pericytes (A488+ or FITC+). (C) Gating for endothelial cells (tdTomato+). (D) Number of events detected and percent of events relative to parent population (All Events) or previous gate.

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