Supplementary Figure 2: Quantification of blood-brain barrier biotin permeability. | Nature Neuroscience

Supplementary Figure 2: Quantification of blood-brain barrier biotin permeability.

From: Profiling the mouse brain endothelial transcriptome in health and disease models reveals a core blood–brain barrier dysfunction module

Supplementary Figure 2

Quantified pixel intensity of biotin:streptavidin-alexa-488 fluorescence on brain/spinal cord tissue sections from seizure, EAE, stroke and TBI disease models and corresponding controls during acute, subacute and chronic timepoints. Biotin was intravenously perfused in anesthetized mice. Data is presented as median fold change of control with arbitrary units (AU). Box plot: 25th–75th percentile box, minimum-maximum whiskers. Statistical test: t test (unpaired, parametric, equal standard deviation, two-tailed), * P-value<0.05, ** P-value<0.01, *** P-value<0.001, **** P-value<0.0001. n=number of mice (Control/Acute/Subacute/Chronic): Seizure: 5/3/4/4, EAE: 4/4/5/5, Stroke: 9/3/3/3, TBI: 8/3/3/3.

Back to article page