Supplementary Figure 3: Quantification of blood-brain barrier fibrinogen permeability.
A-H) Representative images of brain/spinal cord sections immunostained for the endogenous serum component fibrinogen (red) and vessel marker CD31 (green) in control and subacute mice. Fibrinogen is increased in seizure, EAE, stroke and TBI disease models (B,D,F,H) compared to controls (A,C,E,G). B/D/F/H-prime) Higher magnification of boxed regions in subacute showing colocalization of fibrinogen with CD31+ vessels (arrowheads highlight examples). Scale bars: 100 microns; 200 microns in E and G. n=number of independent experiments: Seizure: 2, EAE:2, Stroke: 3, TBI: 4. I) Quantified pixel intensity of fibrinogen immunostaining with alexa-594 fluorophore on tissue sections from seizure, EAE, stroke and TBI disease models and corresponding controls during the subacute timepoint. Data is presented as median fold change of control with arbitrary units (AU). Box plot: 25th-75th percentile box, minimum-maximum whiskers. Statistical test: t test (unpaired, parametric, equal standard deviation, two-tailed), ** P-value<0.01, *** P-value<0.001. n=number of mice (Control/Subacute): Seizure: 3/4, EAE: 4/3 Stroke: 4/4, TBI: 3/3.
