Supplementary Figure 3: D1 receptor expression in basal amygdala.
From: State-specific gating of salient cues by midbrain dopaminergic input to basal amygdala
a, A cross of a red fluorescent reporter mouse with a D1 receptor Cre line (left) or a D2 receptor Cre line (right) provided initial evidence of dense D1 receptor mRNA expression and sparse D2 receptor mRNA expression across both anterior basal amygdala (aBA) and posterior basal amygdala (pBA) neurons, in sagittal sections (data modified from Allen Institute Brain Atlas; D1 receptor: image 3 [http://connectivity.brain-map.org/transgenic/experiment/81034295]; D2 receptor: image 1 [http://connectivity.brain-map.org/transgenic/experiment/100146223]). Because reporter fluorescence could reflect transient receptor expression during development, we also assessed mRNA expression levels in BA neurons using the Allen Brain Atlas (panel b). Scale bar = 0.5 mm. b, D1 receptor mRNA expression (left) and D2 receptor mRNA expression (right) in sagittal sections of BA. Note that expression of the D1 receptor was evident throughout anterior and posterior regions of BA, while D2 receptor expression was largely not detectable in BA (Allen Institute Brain Atlas; D1 receptor: image 3 [http://mouse.brain-map.org/experiment/show/71307280]; D2 receptor: image 3 [http://mouse.brain-map.org/experiment/show/81790728]). Scale bar = 0.5 mm. c, Left: mCherry-labeled VTA dopaminergic axons target BA but not lateral amygdala (LA) (for details, see Fig. 2a). Middle and right: coronal sections demonstrating detectable expression of D1 receptor mRNA (middle, arrow) but not of D2 receptor mRNA (right, arrow) in BA (Allen Institute Brain Atlas; D1 receptor: image 24 [http://mouse.brain-map.org/experiment/show/352]; D2 receptor: image 26 [http://mouse.brain-map.org/experiment/show/357]). Scale bar = 0.5 mm.
