Supplementary Figure 4: Implant locations for VTA^DA→BA fiber photometry experiments.

a, Location of photometry fiber implants from all GCaMP6s photometry mice (horizontal colored lines) with basal amygdala (BA) implants, determined using post-hoc histology (lenses are depicted at scale): 8 mice with bilateral BA implants targeted either to anterior BA (aBA) or posterior BA (pBA) regions, and mice used for two GFP control experiments. Photometry data from these mice are shown in Fig. 2c–e, Fig. 3c–i, Supplementary Figs. 5–9. Coronal sections and coordinates relative to Bregma are based on Paxinos and Franklin’s mouse brain atlas (4^th edition). ‘X’ marks a fiber implant excluded from subsequent analyses due to mis-targeting. b, Location of photometry fiber implants from all BA implanted axon-targeted GCaMP6s photometry mice for sham-satiation and home cage satiation experiments (horizontal colored lines) determined from post-hoc histology (lenses are depicted at scale): 8 mice. Photometry data from these mice are shown in Figs. 2f–h and 3j–l. Coronal sections and coordinates relative to Bregma are based on Paxinos and Franklin’s mouse brain atlas (4^th edition). c, Location of photometry fiber implants from all GCaMP6s photometry mice for comparing nucleus accumbens (NAc) vs. BA responses (horizontal colored lines) determined from post-hoc histology (lenses are depicted at scale): 4 mice with ipsilateral BA and NAc implants. Photometry data from these mice are shown in Fig. 4b–d. Coronal sections and coordinates relative to Bregma are based on Paxinos and Franklin’s mouse brain atlas (4^th edition). d, Location of photometry fiber implants from all dLight1.1 photometry mice for comparing NAc vs. BA responses (horizontal colored lines) determined from post-hoc histology (lenses are depicted at scale): 4 mice with ipsilateral BA and NAc implants. Photometry data from these mice are shown in Fig. 4e–g. Coronal sections and coordinates relative to Bregma are based on Paxinos and Franklin’s mouse brain atlas (4^th edition). e, Location of photometry fiber implants from all BA implanted dLight1.1 photometry mice for Chrimson optogenetic stimulation of VTA dopamine axon terminals (horizontal colored lines) determined from post-hoc histology (lenses are depicted at scale): 4 mice. Photometry data from these mice are shown in Fig. 4i. Coronal sections and coordinates relative to Bregma are based on Paxinos and Franklin’s mouse brain atlas (4^th edition).