Supplementary Figure 9: VTA^DA→BA axon cue responses are similar across anterior BA and posterior BA.

a, Mean timecourse during trials involving a reward cue (RC), a neutral cue (NC), or an aversive cue predicting unavoidable air puff (AC-Un), for anterior BA (aBA) and posterior BA (pBA) recordings. Error bars: s.e.m. across 10 sessions from 6 mice. b, Cue response magnitude of VTA^DA→BA recordings in aBA or pBA (mean ± s.e.m., n = 10 sessions from 6 mice). **p = 0.002, two-sided Wilcoxon sign-rank. c, Mean timecourse during trials involving a reward cue (RC), a neutral cue (NC), or an aversive cue predicting unavoidable tail shock (AC-Un), for aBA and pBA recordings. Error bars: s.e.m. across 11 sessions from 8 mice. d, Cue response magnitude of VTA^DA→BA axon recordings from anterior vs. posterior BA (mean ± s.e.m., n = 11 sessions from 8 mice). *** p = 0.0009, two-sided Wilcoxon sign-rank. e, Mean response timecourses of VTA^DA→BA axons during training and following completion of training on avoidable quinine task, from aBA (left) or pBA (right). Response timecourse of Ensure delivery related activity was obtained by subtracting a monoexponential fit of the cue response. The window used for analysis of Ensure delivery responses is indicated by a blue bar. Error bars: s.e.m. across 8 “during training” and 6 “trained” mice. Z: Z-score. f, Cue and outcome response magnitudes of VTA^DA→BA axon recordings from aBA or pBA during training (mean ± s.e.m., n = 8 mice) vs. following training (n = 6). During training, no differences were found between aBA and pBA (p > 0.05, Wilcoxon rank-sum). Following training, similar to Supplementary Fig 9b,d, RC response magnitudes were greater in aBA. * p = 0.03, two-sided Wilcoxon rank-sum. g, Mean response timecourses of VTA^DA→BA axons during first sessions of training with air puff (top) or tail shock (bottom) in aBA (left) and pBA (right). Response timecourse of aversive outcome delivery-related activity was obtained by subtracting a monoexponential fit of the cue response. The window used for analysis of air puff or tail shock delivery responses is indicated by a blue bar. Error bars: s.e.m. across 6 mice. h, Outcome response magnitudes of VTA^DA→BA axon recordings from aBA or pBA during first day of air puff training (top) or first day of tail shock training (bottom). Responses to delivery of air puff or tail shock were not different between aBA and pBA (mean ± s.e.m., n = 6 mice), two-sided Wilcoxon rank-sum.