Supplementary Figure 12: Changes in pupil area and locomotion across states do not account for observed changes in BA activity in mice trained with cue predicting rewards and cues predicting unavoidable tail shock.

a, Histograms of pupil areas in the 2 s preceding cues during hungry and sated states from an example session, before (left) and after (right) matching trials for pupil area (see Methods). Right: mean pupil area in the 2 s preceding a cue during hungry and sated states (normalized to maximum pupil area during that entire session across both states). Mean ± s.e.m. across 9 sessions from 4 mice. n.s.= not significant, p = 0.054, two-sided Wilcoxon sign-rank. b, Histograms of locomotion in the 2 s preceding cues during hungry and sated states from an example session before (left) and after (right) matching trials for locomotion. Right: mean locomotion in the 2 s preceding a cue during hungry and sated states (normalized to maximum locomotion during that entire session across both states). Mean ± s.e.m. across 9 sessions from 4 mice. ** p = 0.0039, two-sided Wilcoxon sign-rank. c, Comparison of cue responses (mean ± s.e.m.) of BA neurons that were significantly responsive in the hungry state. Attenuation of cue responses following satiation persisted after matching pupil area distributions across states. Sample size for RC: n = 98 activated neurons and n = 83 suppressed neurons; for AC-Un: n = 136 activated neurons and n = 82 suppressed neurons; for NC: n = 98 activated neurons and n = 69 suppressed neurons. For all comparisons: *** p < 0.0001, two-sided Wilcoxon sign-rank. d, Comparison of cue responses (mean ± s.e.m.) of BA neurons that were significantly responsive in the hungry state. Attenuation of cue responses following satiation persisted after matching locomotion distributions across states. Sample sizes are identical to panel c. For all comparisons: *** p < 0.0001, two-sided Wilcoxon sign-rank. e, Comparison of cue responses (mean ± s.e.m.) of BA neurons that were significantly responsive in the sated state. Enhancement of cue responses following satiation persisted after matching pupil area distributions across states. Sample size for RC: n = 79 activated neurons and n = 21 suppressed neurons, for AC-Un: n = 108 activated neurons and n = 38 suppressed neurons, for NC: n = 89 activated neurons and n = 26 suppressed neurons. Original AC-Un activated response magnitude, hungry vs. sated: ** p = 0.0031. Pupil-matched AC-Un activated response magnitude hungry vs. sated: *** p < 0.0001. Original AC-Un suppressed response magnitude, hungry vs. sated: * p = 0.013. Pupil-matched AC-Un suppressed response magnitude hungry vs. sated: ** p = 0.0029. Original NC activated response magnitude hungry vs. sated: *** p < 0.0001. Pupil-matched NC activated response magnitude,, hungry vs. sated: *** p < 0.0001. Two-sided Wilcoxon sign-rank. f, Comparison of cue responses (mean ± s.e.m.) of BA neurons that were significantly responsive in the sated state. Enhancements of cue responses following satiation persisted after matching locomotion distributions across states. Sample sizes are identical to panel e. Original AC-Un activated response magnitude, hungry vs. sated: ** p = 0.0031. Locomotion-matched AC-Un activated response magnitude hungry vs. sated: *** p < 0.0001. Original AC-Un suppressed response magnitude, hungry vs. sated: * p = 0.013. Locomotion-matched AC-Un suppressed response magnitude, hungry vs. sated: * p = 0.017. Original NC activated response magnitude, hungry vs. sated: *** p < 0.0001. Locomotion-matched NC activated response magnitude, hungry vs. sated: p = 0.1. Two-sided Wilcoxon sign-rank.