Supplementary Figure 4: PCE does not modify TH+ cell number in male offspring.
From: Prenatal THC exposure produces a hyperdopaminergic phenotype rescued by pregnenolone
(a) Confocal image of anti-TH immunostaining in control rat brain. (b) Boxed region in a). (c) Confocal image of anti-TH immunostaining in PCE rat brain. (d) Boxed region in c). n= 12 images per animal were acquired independently, with similar results obtained. (e) Mean TH+ cell densities of the VTA ROIs showing no significant difference between treatment groups (P=0.665, ns; two-sided Mann-Whitney U-test). (f) Mean TH pixel intensities of the VTA ROI showing no significant difference between treatment groups (P=0.885, ns; two-sided Mann-Whitney U-test). Data in (e, f) are represented with floating bars (min to max, line at median) with single values (each circle represents n= 1 rat).
