Extended Data Fig. 8: Additional conformational stability assay (CSA) data for α-syn species in brain homogenates from inoculated TgM83 mice.
From: α-Synuclein strains target distinct brain regions and cell types
a) CSA for PSyn aggregates in clinically ill TgM83 mice inoculated with either the S or NS fibril-derived strains (third passage). Representative PSyn immunoblots and the resultant GdnHCl50 values are shown. The PSyn aggregates in mice inoculated with the NS fibril-derived strain are significantly more stable (**P = 0.0091). Data is mean ± s.e.m (n = 10 mice for the S strain and n = 7 mice for the NS strain). b) CSA for PSyn aggregates in clinically ill TgM83 mice inoculated with either MSA- or M83+/+ brain extract (first passage). Representative PSyn immunoblots and the resultant GdnHCl50 values are shown. Data is mean ± s.e.m (n = 4 for MSA-inoculated mice, n = 6 for M83+/+-inoculated mice). c) CSA for PSyn aggregates in clinically ill TgM83 mice inoculated with either the MSA- or M83+/+-derived strains (second passage). Representative PSyn immunoblots and the resultant GdnHCl50 values are shown. The PSyn aggregates in mice inoculated with the M83+/+-derived strain are significantly more stable (*P = 0.038). Data is mean ± s.e.m (n = 6 for both groups of inoculated mice). All P values were obtained using a two-tailed t-test.
