Extended Data Fig. 4: Altered gamma synchronization in epileptic mice.
From: Breakdown of spatial coding and interneuron synchronization in epileptic mice
a. Gamma power throughout CA1 and DG. Epileptic mice had reduced gamma power in the hilus of DG (2-way RM ANOVA, FGroupXRegion(6,48) = 5.26, P < 0.001, Hilus: P < 0.001). b. Gamma coherence between each channel pair along the recording probe in control (left) and epileptic (middle) mice. Right, p-value matrix for each pair of recording sites (unpaired t-tests, uncorrected for multiple comparisons). c. Phase locking to CA1 gamma for each interneuron in CA1 of control and epileptic mice. Each dot represents one interneuron and the data is double plotted for visualization. Closed circles were significantly phase locked (Raleigh test, P < 0.05) and open circles were not. d. Phase locking to CA1 gamma for each interneuron in DG of control and epileptic mice. e. Mean phase modulation (r) of CA1 interneurons to CA1 gamma. Interneurons in epileptic mice had increased phase modulation (n = 69 Control cells, n = 57 Epileptic cells, Mann-Whitney test, P < 0.001). f. Mean phase modulation (r) of DG interneurons to CA1 gamma. Interneurons in epileptic mice had no change in phase modulation (n = 34 Control cells, n = 41 Epileptic cells, Mann-Whitney test, P = 0.42). g. Rose plot of preferred firing phases of significantly phase locked CA1 interneurons. There were no differences between interneurons in control and epileptic mice (Kuiper circular test, P > 0.05). h. Rose plot of preferred firing phases of significantly phase locked DG interneurons. The distribution of preferred phases in control and epileptic mice were different (Kuiper circular test, P < 0.001). i. Aggregate inhibition of all interneurons in CA1 and DG relative to CA1 gamma phase (2-way RM ANOVA, CA1: FGroupXPhase(17,136) = 1.22, P = 0.25; DG: FGroupXPhase(17,136) = 2.196, P = 0.007). j. Pearson’s correlation between CA1 and DG aggregate inhibition relative to gamma. No difference was found between epileptic and control animals (Unpaired t-test, P = 0.19). All data in this figure came from 5 animals per group. Error bars represent 1 S.E.M. *P < 0.05, ***P < 0.001, ns: not significant.
