Extended Data Fig. 4: Aged plasma induces lipid droplet formation in BV2 cells. | Nature Neuroscience

Extended Data Fig. 4: Aged plasma induces lipid droplet formation in BV2 cells.

From: Lipid-droplet-accumulating microglia represent a dysfunctional and proinflammatory state in the aging brain

Extended Data Fig. 4

a, Representative micrographs of BODIPY+ staining and of phagocytosis of of pHrodo red Zymosan in BV2 cells treated with 5% plasma from young (3-months) and aged (18-months) mice for 12 hours. Scale bars, 5 μm. b, Quantification of BODIPY+ staining in BV2 cells treated with young and aged plasma. c,d, Quantification of Zymosan uptake in BV2 cells treated with young and aged plasma (c), and in aged plasma treated BODIPY-low and BODIPY-high cells (d). Statistical tests: two-sided Student’s t-test. Error bars represent mean ± SD. *P< 0.05, ***P< 0.001.

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