Extended Data Fig. 3: Reward size reduction resembles post-reward DA neuron inhibition.
From: Temporally restricted dopaminergic control of reward-conditioned movements
(a) (Top) Schematic illustration the test using reduced reward size. The reward was reduced from 5 µL in block 1 to 2 or 3 µL in block 2, then reinstated to 5 µL in block 3. (Middle and bottom) Mean lick rate versus time for a reduced reward of 2 and 3 µL (n = 7 mice). Black and green lines represent trial blocks 1 and 2, respectively. (b) Anticipatory lick probability in each of the three trial blocks for the two reduced reward conditions (n = 7 mice, two-way RM ANOVA, reward size effect: F1,6 = 34.2, P = 0.001, trial block effect: F2,12 = 27, P < 0.0001. Post-hoc Sidak’s test: ****P < 0.0001. (c) Mean normalized number of anticipatory licks per animal as a function of trial number for the two reduced reward size conditions. Left plot shows data aligned to the start of block 2, and right plot shows data aligned to the start of block 3. (d) Fractional change in anticipatory licking probability as a function of reward size in block 2 (n = 7 mice, one-way RM ANOVA, F = 37.6, P < 0.0001). Post-hoc Tukey’s test: 2 vs 3 µL P = 0.002, 2 vs 5 µL P = 0.0004, 3 vs 5 µL P = 0.09. Data are expressed as mean ± SEM.
