Extended Data Fig. 2: Effect of post-reward VTA DA neuron inhibition on anticipatory licking.
From: Temporally restricted dopaminergic control of reward-conditioned movements
(a) (Left) Trial structure of Pavlovian conditioning task with post-reward VTA DA inhibition. (Right) Mean lick rate versus time for NpHR3.0 (n = 18) and YFP (n = 14) expressing animals undergoing post-reward inhibition. Black and green lines represent block 1 (trials 1 – 40, laser off) and block 2 (trials 41 – 80, laser on), respectively. (b) (Left) During post-reward inhibition, anticipatory lick number was significantly reduced in the laser block compared to controls (n = 18 eNpHR3.0+ and 14 YFP+ mice, two-way RM ANOVA, group effect: F1,30 = 2.4, P = 0.13, trial block effect: F2,60 = 24.8, P < 0.0001). Post-hoc Sidak’s test: **P = 0.003. (Right) Anticipatory lick onset time was not significantly altered in the laser block relative to controls (two-way RM ANOVA, group effect: F1,30 = 0.0001, P = 0.99, trial block effect: F2,60 = 9.2, P = 0.0003). (c) Mean number of anticipatory licks per animal (n = 18 eNpHR3.0+ and 14 YFP+ mice) as a function of trial number, for post-reward laser stimulation. Data are normalized to the mean lick count in the first trial block corresponding to laser off. Data are aligned to the start of the third trial block. Data are expressed as mean ± SEM.
