Extended Data Fig. 5: Blocking cap-dependent translation in LA CamK2α principal neurons. | Nature Neuroscience

Extended Data Fig. 5: Blocking cap-dependent translation in LA CamK2α principal neurons.

From: Cell-type-specific drug-inducible protein synthesis inhibition demonstrates that memory consolidation requires rapid neuronal translation

Extended Data Fig. 5

a) Alternate strategy of blocking translation in CamK2α principal neurons in LA using cre-tet regulated synthetic micro-RNA targeted against eIF4E. Col1a1.TRE.GFP.shmir4E mice were bilaterally injected in the lateral amygdala with AAV1.CamK2α.Cre and AAV9.DIO.tTA, and placed off dox diet for 10 days before training. b) eIF4E protein level was significantly decreased in GFP+ neurons that express shmir4E. Two-way ANOVA with Bonferroni’s post-hoc test. Genotype X GFP interaction: F(1,311) = 32.29, ****p<0.0001; GFP: F(1,311) = 45.32, ****p<0.0001. c) CamK2α 4Ekd mice learned the association between CS and US during training. RM Two-way ANOVA with Bonferroni’s post-hoc test. CS: F(2,14) = 17.54, ***p=0.0002. d) Cued LTM was severely impaired across all three CS presentations. n=8 per group; RM Two-way ANOVA with Bonferroni’s post-hoc test. F(10,20) = 15.65, **p=0.0027. d) Mean cTC LTM was significantly impaired in CamK2α 4Ekd mice compared to wildtype (***p<0.001). n = 8 per group; Unpaired t-test. Data are presented as ± SEM.

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