Extended Data Fig. 6: Characterization of mice expressing phosphomutant eIF2α in LA CamK2α principal neurons. | Nature Neuroscience

Extended Data Fig. 6: Characterization of mice expressing phosphomutant eIF2α in LA CamK2α principal neurons.

From: Cell-type-specific drug-inducible protein synthesis inhibition demonstrates that memory consolidation requires rapid neuronal translation

Extended Data Fig. 6

a) eIF2α phosphorylation at S51 was significantly reduced in GFP+ neurons in CamK2α.eIF2α (A/A) mice compared to GFP- neurons, as well as GFP+ neurons in CamK2α.WT mice (****p<0.001). (n = 60–74 per group, 3 animals); One-way ANOVA with Bonferroni’s post-hoc test. F (2, 154) = 1055, ****p<0.0001. b) Representative motion traces from the open field test for CamK2α.WT, CamK2α.eIF2α(A/+) and CamK2α eIF2α (A/A) mice. c) In the open field test, CamK2α.eIF2α (A/A) mice acclimated to the novel environment and had comparable spontaneous locomotion compared to the CamK2α.WT mice and CamK2α. eIF2α (A/+) mice. RM One-way ANOVA. d) Bar graphs representing thigmotaxis, i.e. %time spent in center compared to total distance traversed in the open field arena for the three groups. One-way ANOVA. f) In the elevated plus maze, CamK2α.eIF2α (A/A) mice spent a significantly higher duration in the open arm compared to CamK2α WT mice (g) (*p<0.05) indicating anxiety like behavior, even though they made equivalent entries to the open arm. One-way ANOVA with Bonferroni’s post-hoc test. F(2,17) = 3.775, *p=0.0440. Data are presented as +/- SEM.

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