Extended Data Fig. 6: Alignment of the dArc1 and mature HIV-1 structures.
From: Structures of virus-like capsids formed by the Drosophila neuronal Arc proteins
a, CA from the five-fold capsomere of the dArc1 capsid aligned with CA from the HIV-1 CA pentamer from mature virions (PDB ID: 5MCY) 5by either CANTD (HIV-1; light blue) or CACTD (HIV-1; dark blue). The HIV-1 CANTD is composed by 7 helical segments and an N-terminal β-Hairpin. α1-α4 in dArc CANTD correspond to α2-α4 and α7 in the HIV-1 CANTD. The CACTD fold is well conserved. b-c, The arrangement of CACTD at the two and three-fold interfaces in the dArc capsid is similar to the arrangement at the corresponding positions in mature HIV-1 (PDB ID: 5MCX)40. The HIV-1 two-fold CACTD interface is constituted by a short 310 helical segment and α9 which align with dArc α5 and α7, respectively. Mutations in HIV-1 CA α9 are known to disrupt virus assembly and maturation41 40. Mattei, S., Glass, B., Hagen, W. J. H., Kräusslich, H.-G. & Briggs, J. A. G. The structure and flexibility of conical HIV-1 capsids determined within intact virions. Science 354, 1434–1437 (2016). 41. Joshi, A., Nagashima, K. & Freed, E. O. Mutation of dileucine-like motifs in the human immunodeficiency virus type 1 capsid disrupts virus assembly, gag-gag interactions, gag-membrane binding, and virion maturation. J. Virol. 80, 7939–7951 (2006).
