Extended Data Fig. 3: Pharmacological responsiveness of the supSDH astrocytes to 5-HT and NE, and generation of Adra1a^flox/flox mice.

a, Amplitudes of Ca²⁺ increases evoked by bath application of 5-HT (10 or 100 μM) and NE (10 μM) to spinal cord slices (n = 86 cells, 4 slices, 4 mice). b, Effect of adrenergic agonists (n = 68 cells, 3 slices, 3 mice for phenylephrine, n = 60 cells, 3 slices, 3 mice for clonidine, n = 64 cells, 3 slices, 3 mice for isoproterenol) on astrocytic Ca²⁺ levels. Note that the α₁-AR agonist phenylephrine produced Ca²⁺ increases, but the α₂-AR agonist clonidine and the β-AR agonist isoproterenol did not. Data show the mean ± s.e.m. c, Schematic illustration to generate a conditional allele at the Adra1a locus. d, Sequences of ssODNs with 5′- and 3′-homology arms flanking loxP and a restriction site. Asterisks indicates a phosphorothioate bond. e, Immunofluorescence of α_1A-ARs in Adra1a^floxflox and Hes5-CreERT2;Adra1a^floxflox mice with tamoxifen administration. The data are representative of three independent experiments. Scale bar, 20 µm.