Extended Data Fig. 8: α1A-AR-knockdown of Hes5-negative astrocytes in deeper laminae of the SDH. | Nature Neuroscience

Extended Data Fig. 8: α1A-AR-knockdown of Hes5-negative astrocytes in deeper laminae of the SDH.

From: Spinal astrocytes in superficial laminae gate brainstem descending control of mechanosensory hypersensitivity

Extended Data Fig. 8

Schematic illustration of the Cre-OFF system to knockdown α1A-ARs in Cre-negative (Hes5-negative) astrocytes. AAV-gfaABC1D-shRNA (shScramble or shAdra1a)FLEX was microinjected unilaterally into the SDH of Hes5-CreERT2 mice. Assessment of mechanical hypersensitivity after intraplantar injection of capsaicin was conducted 7 days after the last tamoxifen treatment.

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