Extended Data Fig. 9: Distribution of Hes5+ SDH astrocytes after peripheral nerve injury.

a, Schematic timeline for tamoxifen treatments and fixation. Mice were injected with tamoxifen once a day for 5 days [days 5 to 9 or days 12 to 16 after peripheral nerve injury (PNI)] and were fixed 7 days after the last tamoxifen treatment. b, tdTomato expression in the SDH of Hes5-CreERT2;Rosa-tdTomato mice after PNI. c, Immunostaining of SOX9 in the ipsilateral SDH of Hes5-CreERT2;Rosa-tdTomato mice after PNI. Note that cell-type specificity and the expression pattern of tdTomato+ cells were not changed after PNI. In b and c, the data are representative of three independent experiments. Scale bars, 200 μm (c) and 500 μm (b).