Extended Data Fig. 4: Representations of serial-sectioning reconstructions of active zones with exocytic pits.
From: Synaptic vesicles transiently dock to refill release sites
a, Graphical depictions of serial-sectioned active zones containing exocytic pits, from the experiments described in Fig. 2. b, Sizes of active zones from the data set shown in Fig. 2. Area was calculated as the product of the longest 2-D length of active zone in a 2-D profile from that synapse, the number of sections containing the active zone, and section thickness (that is, as the area of a rectangle). 1.2 mM, no stim, n = 62; 1.2 mM, stim, n = 68; 2 mM, no stim, n = 64; 2 mM, stim, n = 66; 4 mM, no stim, n = 65; 4 mM, stim, n = 64 reconstructed active zones. Error bars indicate median and interquartile range. c, Density of docked vesicles in the active zone, from the experiments shown in Fig. 2. Calculated as the total number of docked vesicles from a given sample x10000, divided by the sum of the total area of active zones from that sample. d, Density of exocytic pits in the active zone, from the experiments shown in Fig. 2. Calculated as the total number of pits from a given sample x10000, divided by the sum of the total area of active zones from that sample. e, Same data shown in b, sorted by whether the active zone contained an exocytic pit or not. See Supplementary Table 1 for full pairwise comparisons and summary statistics.
