Extended Data Fig. 1: Validation and characterization of GiD system.
a, (left) RT-PCR analysis of diphtheria toxin receptor(DTR) in hippocampus of Gcon and GiDm. (right) RT-PCR analysis of DTR in cultured astrocytes from Gcon and GiD (GiDc). GAPDH, internal control. Experiments were repeated more than twice. b, Measuring passive conductance in astrocytes of Gcon and GiDs. c, Experimental procedures of GiD mice, which GFAP-CreERT2 crossed with iDTR mice. Tam, tamoxifen, 100 mg/kg/day, 5 days; DT, 50 µg/kg/day, 2 days. d, GFAP immunostaining in brain regions of frontal lobe containing cortex, hippocampus, striatum and amygdala of Gcon and GiD mice. Ctx, cortex; Hipp, hippocampus; Str, striatum; Amyg, amygdala. e, Experimental procedures for PI staining in GiDm, CiD mice and KA-injected seizure model mice. DT, 50 µg/kg/day, 2 days; KA, kainic acid, 25 mg/kg. f, PI staining in GiDm, CiD and seizure model mice. g, h, MTT assay in DT-treated GiD astrocyte. DT, 1 ug/ml, 5 days; 3-MA, 3-Methyladenine, 0.5 mM (g), Baf A1, bafilomycin A1, 4 µM (h). Data are presented as mean ± SEM. *P < 0.05; NS: not significant. Additional statistical details are provided in Supplementary Table 1.
