Extended Data Fig. 9: Impaired forepaw RAM mechanosensitivity underlies 25Hz vibration perceptual deficits in Ush2a-/- mice.
From: USH2A is a Meissner’s corpuscle protein necessary for normal vibration sensing in mice and humans
a, Box and whisker plot of median first lick latencies of Ush2a+/+ (n=6) and Ush2a-/- (n=5) mice at 5Hz, 25Hz and 100Hz frequency vibration, whisker indicates max and min values, box indicates 1st 2nd (median) and 3rd quartiles. b PSTHs of first lick responses to 25Hz 12mN vibration stimuli from all behaving Ush2a+/+ and Ush2a-/- trials. Peak first lick Hit responses above False lick rates indicate first responses of mice 25Hz stimuli. Ush2a-/- mice did not detect this stimulus c Spike timing raster plots of individual forepaw RAMs and SAMs recorded in response to the first 20 cycles of a 25Hz vibration stimulus in the ex vivo forepaw skin nerve preparation of Ush2a+/+ and Ush2a-/- mice. Each row represents one trial from a single afferent 1-3 trials per recording (left Ush2a+/+ RAM n = 4/11, SAM 2/10 afferents; right Ush2a-/- RAM n = 1/10, SAM 1/11 afferents). d, PSTHs of mean first lick responses to 60mN 25Hz vibration stimuli from all behaving Ush2a+/+ and Ush2a-/-, both genotypes detected this stimulus. e, Spike timings of individual RAMs and SAMs recorded in the ex vivo forepaw skin nerve preparation in Ush2a+/+ and Ush2a-/- mice in response to the first 20 cycles of 60mN 25Hz stimuli (left Ush2a+/+ RAM n = 10/11, SAM n = 10/10; right Ush2a-/- RAM n = 8/10, SAM n = 10/11). Data = mean ± s.e.m.
