Extended Data Fig. 3: USH2A immunolabelling in cutaneous Meissner corpuscles.
From: USH2A is a Meissner’s corpuscle protein necessary for normal vibration sensing in mice and humans
a, USH2A (green) and NF200 (red) immunolabelling of dorsal root ganglia neurons. a’ USH2A channel only. a’’ NF200 channel only. A’’’ RNA scope probe for Ush2a mRNA shows no labelling in the DRG (green) a second probe for NF200 mRNA (red) shows large sensory neurons labeled. The Ush2a RNA labels Meissner corpuscle cells in the skin (green). b NF200 (red), and S100 (cyan) immunolabelling of Meissner corpuscles in the sectioned paw glabrous skin. b’ USH2A channel. b’’ NF200 channel. b’’’ S100 . c, USH2A (green) and NF200 (red) immunolabelling of deep nerve branches in whole mounted paw skin show an absence of USH2A in nerve bundles. c’ USH2A channel only. c’’ NF200 channel. d, USH2A (green) and NF200 (red) immunolabelling of Meissner corpuscles in the sectioned paw glabrous skin from an Ush2a-/- mouse show an absence of USH2A labelling. d’ USH2A channel only. d’’ NF200 channel only.
